In Vitro Reconstituted Ubiquitylation Assays for Mechanistic Studies of Cullin-RING Ligases

Introduction Cullin-RING ligases (CRLs) comprise the largest family of E3 ubiquitin ligases and use hundreds of substrate receptors to regulate protein stability and cell signaling. Unlike HECT and RBR ligases, which form catalytic E3–ubiquitin intermediates, CRLs are modular, multi-subunit RING ligases assembled around one of seven canonical mammalian Cullin proteins.  Each Cullin serves as a […]