Custom Services Built for UPS Biology and Degrader Mechanisms
Mechanism-first. Reproducible by design.
- CRO dedicated to ubiquitin proteasome biology and protein degradation
- Assays designed to reflect UPS biology and degrader mechanisms, ensuring biological relevance
- Run by PhD scientists with deep expertise in E3 ligase biology, substrate design, and degrader MoA
E3 Ligase Assay Development
Assay design and development for pathway-relevant E3 ligases in targeted protein degradation
Pathway-Aligned Readouts: Ternary complex formation, in vitro E1-E2-E3-substrate ubiquitylation cascade
Ligase-Centric Assay Design: Assay optimized for AlphaLISA, HTRF, FP and Fluorescence
Flexible System Integration: Ligase-substrate pairing and degrader mechanism evaluation
Deliverables:
- Assay-ready plate or validated protocol
- Signal optimization data report
- Optional pilot-scale production for HTS integration
Protein Expression and Purification
Rapid screening of bacterial expression conditions to produce high-yield UPS proteins
Expression Flexibility: Tagged/untagged, HTS-ready, and assay-optimized formats
Yield Optimization: 48 parameter parallel scouting expression screening for yield, bioactivity and reproducibility
Scalable Expression: Up to 40L in E. coli of E3 ligases, adaptors, substrates, DUBs, or multi-subunit complexes
Deliverables:
- QC-tested bioactive protein aliquots
- Expression/purification summary report
Ready to discuss your project?
Connect directly with Trilogie scientists to discuss your targets, experimental goals, and project requirements.
All services performed at our Worcester, MA facility
What to Expect When Working with Trilogie
Scientist-to-scientist engagement from day one.
You’ll work directly with Trilogie scientists who understand UPS biology
Assay design rooted in mechanism.
Tailored expression and assay development around your ligase, substrate, or degrader strategy
Flexible, milestone-based project execution.
Alignment on key objectives early and adaptable workflows to match the project
Rigorous QC and data transparency.
Every reagent and assay component is validated for activity with documentation provided for each lot
Transparent updates, milestone-based billing.
Progress summaries at defined checkpoints, and invoicing for completed, approved milestones.
Looking for validated UPS reagents?
Browse Trilogie’s catalog of E1, E2, E3 ligases, target proteins, assay kits, and polyubiquitin tools designed for targeted protein degradation research.
FAQs
Can Trilogie work with my preferred E3 ligase, even if it's novel or low-expression?
Yes. We specialize in expression scouting and assay design for both existing and novel E3 ligases, including those with atypical domain structures or low natural abundance. For those proteins in which bacterial expression is an option, our optimization platform screens up to 48 conditions to identify constructs and formats that maximize yield and bioactivity.
For E3 ligases >100 kDa, an alternate expression system will be used.
What’s the typical turnaround time for a protein expression scouting project?
Most expression scouting projects are completed in 4-6 weeks, depending on construct complexity and yield optimization scope. We deliver written reports detailing findings from our expression studies.
Can Trilogie scale successful expression systems for HTS or structural work?
Yes. Once a high-yield condition is identified via the Trilogie UbIQ™ platform, we can scale production to deliver assay-ready aliquots or milligram quantities for cryo-EM or SPR. We’ll include expression protocols and optional tag removal strategies as needed.
How do your assays support degrader mechanism-of-action studies?
Our assays are built around the full UPS cascade (E1–E2–E3–substrate), enabling accurate measurement of ternary complex formation and substrate ubiquitination. We tailor readouts (e.g., AlphaLISA, HTRF, FP and Fluorescence) to your degrader’s biology, supporting MoA validation, not just phenotypic endpoints.
How do you handle reagent handoff and material logistics?
We ship reagents that have gone through our QC process, (proteins, assay plates, or constructs) on dry ice or cold packs, depending on stability requirements. You’ll receive a tracking number, CoA, and any required documentation for material transfer.
Where are services performed, and how do you manage quality?
All services are performed at our Worcester, MA facility, ensuring tight QC control, full traceability, and transparent scientific communication. You’ll work directly with Trilogie scientists throughout your project.