2 μg K6-linked Tetra-Ubiquitin run on 4-12% SDS-PAGE gel under reducing conditions, then visualized with Colloidal Coomassie Blue Stain.

Recombinant Human K6-linked Tetra-Ubiquitin

Ub/Ubl's

Product Code: TUB-047

Verified Applications: Ubiquitin chains exhibit diversity in length, linkage type, and associated cellular functions. K6-linked Tetra-Ubiquitin serves as a valuable reagent in assays involving ubiquitin-binding proteins and as a substrate for ubiquitin-specific deubiquitylating enzymes (DUBs).

Activity: Fully hydrolyzed by the K6-specific LotA-N deubiquitylase.

Purity: 95 %

Concentration: 29 μΜ

For Research Use Only (RUO)

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Note: To maintain product quality, larger quantities may be packaged as multiple smaller aliquots. The 1 mg size is generally supplied in a single vial.

UniProt #: Ub

Predicted Molecular Weight: 34 kDa

Tag: Untagged

Background and Additional Info

Background: K6-linked polyubiquitin consists of multiple ubiquitins linked together between the C-terminal glycine of one ubiquitin and lysine 6 of the next. K6 linkages are less abundant than K48 or K63 chains and are formed by specific E2/E3 enzyme pairs. Functionally, K6 chains are implicated in mitochondrial quality control, mitophagy, DNA damage response, and regulation of protein-protein interactions rather than proteasomal degradation. They can recruit distinct ubiquitin-binding proteins and deubiquitylases (DUBs), producing signaling outcomes unique from other linkage types.

Shipping: The product is shipped with dry ice. Upon receipt, store it immediately at the temperature recommended below.

Storage and Stability: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Aliquot and store ≤ -20°C (stable for 24 months from date of receipt).

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References: Hospenthal, M. K., et al., (2013) Nat Struct Mol Biol 20:555-565. PMID 23563141

Warren, G. D., et al., (2023) Mol Cell 83:105-120.e5. PMID 36538933

Durcan, T.M. and Fon, E.A. (2015) Genes Dev 29(10):989-99. PMID: 25995186

Alternate Names: Human Lys6-linked Tetra-Ubiquitin (K6-Ub₄, Ub₄-K6)

Protein Sequence: MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG