Recombinant Human Miro1

Background: Miro1 (Mitochondrial Rho GTPase 1; RHOT1) is a mitochondrial outer membrane GTPase that plays a central role in regulating mitochondrial trafficking, distribution, morphology, and quality control. Through its N-terminal GTPase domain, EF-hand calcium-binding motifs, and C-terminal GTPase domain, Miro1 coordinates interactions between mitochondria and motor adaptor proteins, enabling calcium-dependent transport of mitochondria along the cytoskeleton. Miro1 is also a key component of the PINK1/Parkin mitophagy pathway, where ubiquitin-mediated modification of Miro1 promotes the arrest of mitochondrial motility and facilitates selective removal of damaged mitochondria. Dysregulation of Miro1 function has been implicated in Parkinson’s disease and other neurodegenerative disorders, making it an important target for studies of mitochondrial quality control, ubiquitylation, and targeted protein degradation. This recombinant Miro1 protein is well suited for biochemical and biophysical studies of mitochondrial homeostasis, E3 ligase activity, and degrader discovery workflows.