Recombinant Human BRD4 Bromodomain Proteins Set (BD1, BD2, & BD1+BD2)

Background: The BRD4 Bromodomain Proteins Set includes recombinant constructs encompassing Bromodomain 1 (BD1; aa 49–170), Bromodomain 2 (BD2; aa 333–463), and the tandem Bromodomains 1 & 2 (BD1+BD2; aa 49–463) of human BRD4 (25 μg of each). These proteins are designed to support biochemical, biophysical, and structural studies of bromodomain-mediated recognition of acetylated lysine residues. The availability of individual and tandem bromodomain formats enables direct assessment of domain-specific binding interactions, inhibitor selectivity, and bromodomain cooperativity. All constructs are site-specifically biotinylated, enabling robust immobilization and capture on streptavidin-coated surfaces for applications including surface plasmon resonance (SPR), biolayer interferometry (BLI), ELISA-based binding assays, pull-down experiments, affinity capture workflows, and other platform technologies requiring oriented protein presentation. Each construct is produced to high purity and quality standards to facilitate assay development, compound screening, and mechanistic investigations. Together, this protein set provides a comprehensive toolkit for advancing BET bromodomain research and therapeutic discovery.