2 μg DCAF16 / DDB1 / DDA1 run on 4-12% SDS-PAGE gel under reducing conditions, then visualized with Colloidal Coomassie Blue Stain.

Active, Recombinant Human DCAF16 / DDB1 / DDA1 Complex

E3 Ligases

Product Code: TE3-112

Verified Applications: DCAF16 / DDB1 / DDA1 is active in ternary complex formation assays using recombinant BRD4 and the degrader molecule KB02-JQ1.

Activity: Verified in Ternary Complex Assay.

Purity: 90 %

Concentration: 5-10 μM

For Research Use Only (RUO)

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UniProt #: Q16531 ; Q9BW61 ; Q9NXF7

Predicted Molecular Weight: DCAF16: 26 kDa / DDB1: 129 kDa / DDA1: 12 kDa

Tag: DCAF16: 8xHis-TEV / DDB1: FLAG-TEV / DDA1: Untagged

Background and Additional Info

Background: DDB1- and CUL4-associated factor 16 (DCAF16) is the substrate recognition subunit of the CRL4-DCAF16 cullin E3 ubiquitin ligase complex. It facilitates the ubiquitylation and subsequent proteasomal degradation of the nuclear protein Spindlin-4 (SPIN4), which may be the only natural substrate for this ligase. Structurally, DCAF16 consists of a compact α-helical bundle and a short C-terminal acidic tail that mediates DDB1 binding. DCAF16 residues Cys177, Cys178 and Cys179 are frequent interaction sites for electrophilic ligands used to target FKBP12, PARP2, CDK4/6, BRD4, and BRD9 for ubiquitylation and subsequent proteasomal degradation, demonstrating broad scope and potential for DCAF16 in the field of Targeted Protein Degradation (TPD).

Shipping: The product is shipped with cold packs or dry ice. Upon receipt, store it immediately at the temperature recommended below.

Storage and Stability: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Aliquot and store ≤ -20°C (stable for 48 months from date of receipt).

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References: Campos M.A., et al. (2025) ACS Chem Biol 20 (2): 479-488. PMID 39882752

Hsia O., et al. (2024) Nature 627 (8002): 204-211. PMID 38383787

Li Y-D., et al. (2024) Nat Chem Biol 20 (12): 1640-1649. PMID 39075252

Zhang X., et al. (2021) Biochemistry 60 (31): 2430-2440. PMID: 33636084

Zhang X., et al. (2019) Nat Chem Biol 15 (7): 737-746. PMID 31209349

Alternate Names: C4orf30

Protein Sequence: MDYKDDDDKGSENLYFQGMSYNYVVTAQKPTAVNGCVTGHFTSAEDLNLLIAKNTRLEIYVVTAEGLRPVKEVGMYGKIAVMELFRPKGESKDLLFILTAKYNACILEYKQSGESIDIITRAHGNVQDRIGRPSETGIIGIIDPECRMIGLRLYDGLFKVIPLDRDNKELKAFNIRLEELHVIDVKFLYGCQAPTICFVYQDPQGRHVKTYEVSLREKEFNKGPWKQENVEAEASMVIAVPEPFGGAIIIGQESITYHNGDKYLAIAPPIIKQSTIVCHNRVDPNGSRYLLGDMEGRLFMLLLEKEEQMDGTVTLKDLRVELLGETSIAECLTYLDNGVVFVGSRLGDSQLVKLNVDSNEQGSYVVAMETFTNLGPIVDMCVVDLERQGQGQLVTCSGAFKEGSLRIIRNGIGIHEHASIDLPGIKGLWPLRSDPNRETDDTLVLSFVGQTRVLMLNGEEVEETELMGFVDDQQTFFCGNVAHQQLIQITSASVRLVSQEPKALVSEWKEPQAKNISVASCNSSQVVVAVGRALYYLQIHPQELRQISHTEMEHEVACLDITPLGDSNGLSPLCAIGLWTDISARILKLPSFELLHKEMLGGEIIPRSILMTTFESSHYLLCALGDGALFYFGLNIETGLLSDRKKVTLGTQPTVLRTFRSLSTTNVFACSDRPTVIYSSNHKLVFSNVNLKEVNYMCPLNSDGYPDSLALANNSTLTIGTIDEIQKLHIRTVPLYESPRKICYQEVSQCFGVLSSRIEVQDTSGGTTALRPSASTQALSSSVSSSKLFSSSTAPHETSFGEEVEVHNLLIIDQHTFEVLHAHQFLQNEYALSLVSCKLGKDPNTYFIVGTAMVYPEEAEPKQGRIVVFQYSDGKLQTVAEKEVKGAVYSMVEFNGKLLASINSTVRLYEWTTEKELRTECNHYNNIMALYLKTKGDFILVGDLMRSVLLLAYKPMEGNFEEIARDFNPNWMSAVEILDDDNFLGAENAFNLFVCQKDSAATTDEERQHLQEVGLFHLGEFVNVFCHGSLVMQNLGETSTPTQGSVLFGTVNGMIGLVTSLSESWYNLLLDMQNRLNKVIKSVGKIEHSFWRSFHTERKTEPATGFIDGDLIESFLDISRPKMQEVVANLQYDDGSGMKREATADDLIKVVEELTRIH

MGHHHHHHHHGSENLYFQGSMGPRNPSPDHLSESESEEEENISYLNESSGEEWDSSEEEDSMVPNLSPLESLAWQVKCLLKYSTTWKPLNPNSWLYHAKLLDPSTPVHILREIGLRLSHCSHCVPKLEPIPEWPPLASCGVPPFQKPLTSPSRLSRDHATLNGALQFATKQLSRTLSRATPIPEYLKQIPNSCVSGCCCGWLTKTVKETTRTEPINTTYSYTDFQKAVNKLLTASL

MGMADFLKGLPVYNKSNFSRFHADSVCKASNRRPSVYLPTREYPSEQIIVTEKTNILLRYLHQQWDKKNAAKKRDQEQVELEGESSAPPRKVARTDSPDMHEDT