2 μg DCAF11 / DDB1 / DDA1 run on 4-12% SDS-PAGE gel under reducing conditions, then visualized with Colloidal Coomassie Blue Stain.

DCAF11 / DDB1 / DDA1

E3 Ligases

Product Code: TE3-099

Verified Applications: DCAF11 / DDB1 / DDA1 is active in ternary complex formation assays using recombinant BRD4 and the degrader molecule RCS-03-104-4.

Activity: Verified in Ternary Complex Assay.

Purity: 95 %

Concentration: 2-10 μM

For Research Use Only (RUO)

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Predicted Molecular Weight: DCAF11: 64 kDa / DDB1: 129 kDa / DDA1: 12 kDa

Tag: DCAF11: N-8xHis-TEV / DDB1: N-FLAG-TEV / DDA1: Untagged

Background and Additional Info

Background: DCAF11 (DDB1- and CUL4-associated factor 11) is an adaptor protein for the CUL4-DDB1 E3 ubiquitin ligase complex, mediating substrate recognition and ubiquitination. It contains WD40 repeats enabling protein–protein interactions and targets diverse substrates for proteasomal degradation, regulating processes such as cell cycle progression, DNA damage response, and chromatin remodeling. DCAF11 influences replication stress responses and transcriptional control by recruiting specific factors to CUL4 complexes. Dysregulation or altered expression of DCAF11 has been linked to impaired genome stability and may contribute to disease states, including cancer. Ongoing studies aim to delineate substrate networks and therapeutic potential of modulating DCAF11 activity.

Shipping: The product is shipped with cold packs or dry ice. Upon receipt, store it immediately at the temperature recommended below.

Storage and Stability: Frozen Dry Ice

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References: Wang K., et al. (2021) Cell Rep 37(6):109987 PMID 34758320

Rongrong L., et al. (2021) Cell Stem Cell 28(4):732-747 PMID 33357405

Zhang X., et al. (2021) J Am Chem Soc 143(13):5141-5149 PMID 33783207

Xue G., et al. (2023) Nat Comm 14(1):7908 PMID 38036533

Sarott R.C., et al. (2023) J Am Chem Soc 145(40):21937-21944 PMID 37767920

Alternate Names: WDR23

Protein Sequence: MDYKDDDDKGSENLYFQGMSYNYVVTAQKPTAVNGCVTGHFTSAEDLNLLIAKNTRLEIYVVTAEGLRPVKEVGMYGKIAVMELFRPKGESKDLLFILTAKYNACILEYKQSGESIDIITRAHGNVQDRIGRPSETGIIGIIDPECRMIGLRLYDGLFKVIPLDRDNKELKAFNIRLEELHVIDVKFLYGCQAPTICFVYQDPQGRHVKTYEVSLREKEFNKGPWKQENVEAEASMVIAVPEPFGGAIIIGQESITYHNGDKYLAIAPPIIKQSTIVCHNRVDPNGSRYLLGDMEGRLFMLLLEKEEQMDGTVTLKDLRVELLGETSIAECLTYLDNGVVFVGSRLGDSQLVKLNVDSNEQGSYVVAMETFTNLGPIVDMCVVDLERQGQGQLVTCSGAFKEGSLRIIRNGIGIHEHASIDLPGIKGLWPLRSDPNRETDDTLVLSFVGQTRVLMLNGEEVEETELMGFVDDQQTFFCGNVAHQQLIQITSASVRLVSQEPKALVSEWKEPQAKNISVASCNSSQVVVAVGRALYYLQIHPQELRQISHTEMEHEVACLDITPLGDSNGLSPLCAIGLWTDISARILKLPSFELLHKEMLGGEIIPRSILMTTFESSHYLLCALGDGALFYFGLNIETGLLSDRKKVTLGTQPTVLRTFRSLSTTNVFACSDRPTVIYSSNHKLVFSNVNLKEVNYMCPLNSDGYPDSLALANNSTLTIGTIDEIQKLHIRTVPLYESPRKICYQEVSQCFGVLSSRIEVQDTSGGTTALRPSASTQALSSSVSSSKLFSSSTAPHETSFGEEVEVHNLLIIDQHTFEVLHAHQFLQNEYALSLVSCKLGKDPNTYFIVGTAMVYPEEAEPKQGRIVVFQYSDGKLQTVAEKEVKGAVYSMVEFNGKLLASINSTVRLYEWTTEKELRTECNHYNNIMALYLKTKGDFILVGDLMRSVLLLAYKPMEGNFEEIARDFNPNWMSAVEILDDDNFLGAENAFNLFVCQKDSAATTDEERQHLQEVGLFHLGEFVNVFCHGSLVMQNLGETSTPTQGSVLFGTVNGMIGLVTSLSESWYNLLLDMQNRLNKVIKSVGKIEHSFWRSFHTERKTEPATGFIDGDLIESFLDISRPKMQEVVANLQYDDGSGMKREATADDLIKVVEELTRIH

MGHHHHHHHHGSENLYFQGSMGSRNSSSAGSGSGDPSEGLPRRGAGLRRSEEEEEEDEDVDLAQVLAYLLRRGQVRLVQGGGAANLQFIQALLDSEEENDRAWDGRLGDRYNPPVDATPDTRELEFNEIKTQVELATGQLGLRRAAQKHSFPRMLHQRERGLCHRGSFSLGEQSRVISHFLPNDLGFTDSYSQKAFCGIYSKDGQIFMSACQDQTIRLYDCRYGRFRKFKSIKARDVGWSVLDVAFTPDGNHFLYSSWSDYIHICNIYGEGDTHTALDLRPDERRFAVFSIAVSSDGREVLGGANDGCLYVFDREQNRRTLQIESHEDDVNAVAFADISSQILFSGGDDAICKVWDRRTMREDDPKPVGALAGHQDGITFIDSKGDARYLISNSKDQTIKLWDIRRFSSREGMEASRQAATQQNWDYRWQQVPKKAWRKLKLPGDSSLMTYRGHGVLHTLIRCRFSPIHSTGQQFIYSGCSTGKVVVYDLLSGHIVKKLTNHKACVRDVSWHPFEEKIVSSSWDGNLRLWQYRQAEYFQDDMPESEECASAPAPVPQSSTPFSSPQ

MGMADFLKGLPVYNKSNFSRFHADSVCKASNRRPSVYLPTREYPSEQIIVTEKTNILLRYLHQQWDKKNAAKKRDQEQVELEGESSAPPRKVARTDSPDMHEDT