Verified Applications: The Complete Energy Regeneration System has been functionally validated to sustain ATP-dependent enzymatic activity in extended in vitro ubiquitylation assays, enabling robust ubiquitin conjugation and improved reaction performance.
Note: To maintain product quality, larger quantities may be packaged as multiple smaller aliquots. The 1 mg size is generally supplied in a single vial.
Background and Additional Info
Background: The Complete Energy Regeneration System contains a optimized mixture of recombinant enzymes that continuously regenerates ATP during ATP-dependent enzymatic reactions. By recycling ADP and AMP back to ATP through phosphotransfer reactions, the system maintains a stable supply of ATP, minimizes depletion of energy substrates, and supports sustained reaction kinetics over extended incubation periods. The solution is particularly well suited for in vitro ubiquitylation assays, kinase assays, ATPase assays, and other ATP-dependent biochemical applications where consistent ATP concentrations are essential for optimal enzyme activity. Incorporation of an energy regeneration system can improve reaction efficiency, increase product formation, and reduce the need for high initial ATP concentrations, making it a valuable reagent for assay development, mechanistic studies, and high-throughput screening.
Shipping: The product is shipped with dry ice. Upon receipt, store it immediately at the temperature recommended below.
