Active, Recombinant Human FBXO3 / SKP1 Complex

Background: FBXO3 (F-box only protein 3) is the substrate recognition component of an SCF (SKP1–CUL1–F-box) E3 ubiquitin ligase complex that mediates selective ubiquitylation of target proteins involved in immune signaling, inflammation, and protein homeostasis. Through its conserved F-box domain, FBXO3 associates with SKP1 to form the core adaptor module required for assembly with CUL1-RBX1 and subsequent recruitment of ubiquitin-loaded E2 enzymes. FBXO3 has been implicated in the regulation of innate immune responses through the turnover of proteins that modulate inflammatory signaling pathways, making it an emerging target for therapeutic intervention in inflammatory and infectious diseases. This recombinant human FBXO3 / SKP1 complex provides a defined reagent for biochemical and biophysical studies of SCF complex assembly, substrate recognition, E3 ligase function, inhibitor discovery, and targeted protein degradation research.