Active, Recombinant Human FBXO42 / SKP1 Complex

Background: F-box only protein 42 (FBXO42) is a substrate recognition component of the SKP1-CUL1-RBX1 (SCF) E3 ubiquitin ligase complex, where it recruits specific protein substrates for ubiquitylation and subsequent proteasomal degradation. Through its N-terminal F-box domain, FBXO42 binds SKP1 to assemble a functional SCF ligase, while its C-terminal substrate-binding region mediates selective target recognition. FBXO42 has been implicated in the regulation of cellular signaling, DNA damage responses, and protein quality control, although its substrate repertoire and biological functions continue to be actively investigated. Recombinant FBXO42 pre-complexed with SKP1 provides a stable, physiologically relevant substrate receptor complex for biochemical and structural studies of SCF ligase assembly, substrate recognition, inhibitor discovery, and targeted protein degradation (TPD) research.