2 μg of each Ubiquitin K-Only mutant, Ubiquitin NoK, & Ubiquitin WT run on 4-12% SDS-PAGE gel under reducing conditions, then visualized with Colloidal Coomassie Blue Stain.

Ubiquitin K-Only Mutant Proteins Set

Protein Sets

Product Code: TPS-110

Verified Applications: Recombinant Ubiquitin is activated for use in vitro by Recombinant Ubiquitin Activating Enzyme 1 (UBA1). This initial event in the ubiquitin-proteasome cascade requires Mg-ATP and ubiquitin. Appropriate protein concentrations are specific to the application.

Activity: Verified in Ubiquitin Charging Assay.

Purity: 90 %

Concentration: 400 μM

For Research Use Only (RUO)

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Note: To maintain product quality, larger quantities may be packaged as multiple smaller aliquots. The 1 mg size is generally supplied in a single vial.

UniProt #: Ub ; Ub K11 Only ; Ub K27 Only ; Ub K29 Only ; Ub K33 Only ; Ub K48 Only ; Ub K6 Only ; Ub K63 Only ; Ub NoK

Predicted Molecular Weight: Ubiquitin, KX Only: 8.7 kDa / Ubiquitin, NoK: 8.7 kDa / Ubiquitin, WT: 8.6 kDa

Tag: All Untagged

Background and Additional Info

Background: The Ubiquitin K-Only Mutant Proteins Set contains a set of mutant ubiquitin proteins in which only one canonical lysine residue is preserved (K6, K11, K27, K29, K33, K48, or K63), while all other lysines are mutated to arginine. These “lysine (K)-only” mutants restrict ubiquitin chain elongation to a single defined linkage site, enabling precise characterization of ubiquitin chain topology. By limiting polyubiquitin assembly to one lysine position at a time, this kit allows for the determination of the linkage specificity of E2 conjugating enzymes and E3 ubiquitin ligases. These reagents are particularly valuable for identifying the preferred ubiquitin linkage types formed during enzymatic reactions and for validating mechanistic models of ubiquitin signaling. The kit supports systematic interrogation of ubiquitin chain architecture and provides a controlled framework for studying linkage-dependent signaling outcomes. These proteins are well suited for in vitro ubiquitylation assays, enzyme characterization, and studies of ubiquitin linkage biology.

Shipping: The product is shipped with dry ice. Upon receipt, store it immediately at the temperature recommended below.

Storage and Stability: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Store ≤ -70°C (stable for 24 months from date of receipt).

Download the Datasheet

Protein Sequence: MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG

MQIFVKTLTGRTITLEVEPSDTIENVRARIQDREGIPPDQQRLIFAGRQLEDGRTLSDYNIQRESTLHLVLRLRGG

MQIFVRTLTGKTITLEVEPSDTIENVRARIQDREGIPPDQQRLIFAGRQLEDGRTLSDYNIQRESTLHLVLRLRGG

MQIFVRTLTGRTITLEVEPSDTIENVKARIQDREGIPPDQQRLIFAGRQLEDGRTLSDYNIQRESTLHLVLRLRGG

MQIFVRTLTGRTITLEVEPSDTIENVRAKIQDREGIPPDQQRLIFAGRQLEDGRTLSDYNIQRESTLHLVLRLRGG

MQIFVRTLTGRTITLEVEPSDTIENVRARIQDKEGIPPDQQRLIFAGRQLEDGRTLSDYNIQRESTLHLVLRLRGG

MQIFVRTLTGRTITLEVEPSDTIENVRARIQDREGIPPDQQRLIFAGKQLEDGRTLSDYNIQRESTLHLVLRLRGG

MQIFVRTLTGRTITLEVEPSDTIENVRARIQDREGIPPDQQRLIFAGRQLEDGRTLSDYNIQKESTLHLVLRLRGG

MQIFVRTLTGRTITLEVEPSDTIENVRARIQDREGIPPDQQRLIFAGRQLEDGRTLSDYNIQRESTLHLVLRLRGG