2 μg Ubiquitin, N-Cys run on 4-12% SDS-PAGE gel under reducing conditions, then visualized with Colloidal Coomassie Blue Stain.

Recombinant Human Ubiquitin, N-Cys

Ub/Ubl's

Product Code: TUB-118

Verified Applications: Ubiquitin, N-Cys enables site-specific conjugation of fluorophores and other thiol-reactive probes for controlled, homogeneous labeling. It is ideally suited for biochemical assays, ubiquitylation and deubiquitylation studies, FRET applications, and other in vitro ubiquitin pathway analyses while maintaining native ubiquitin functionality.

Activity: Verified in JF646 and AF647 fluorophore conjugation reactions.

Purity: 95 %

Concentration: 50 – 70 μM

For Research Use Only (RUO)

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Note: To maintain product quality, larger quantities may be packaged as multiple smaller aliquots. The 1 mg size is generally supplied in a single vial.

Predicted Molecular Weight: 9.0 kDa

Tag: Untagged

Background and Additional Info

Background: Ubiquitin, N-Cys is a recombinant ubiquitin variant engineered with a cysteine-containing 5 amino acid sequence (MGCGS) appended to the N-terminus of wild-type ubiquitin to enable site-specific conjugation of fluorophores and other thiol-reactive probes. This design supports precise labeling for biochemical assays, imaging, and ubiquitin pathway studies. The construct maintains native ubiquitin functionality while providing a versatile handle for downstream modification. It is well-suited for applications requiring controlled, homogeneous labeling of ubiquitin.

Shipping: The product is shipped with dry ice. Upon receipt, store it immediately at the temperature recommended below.

Storage and Stability: Frozen Dry Ice

Download the Datasheet

Alternate Names: MGCGS-Ubiquitin

Protein Sequence: MGCGSMQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG