Active, Recombinant Human UBE2D3

Background: Ubiquitin-Conjugating Enzyme E2 D3 (UBE2D3) is an E2 ubiquitin-conjugating enzyme that facilitates the transfer of ubiquitin from E1 activating enzymes to substrate proteins. This 147 amino acid protein is almost entirely UBC domain: an N-terminal α-helix for E3 docking, a four-stranded β-sheet, three α-helices and flexible catalytic loops. UBE2D3 forms thioester-linked UBE2D3-ubiquitin conjugates that cooperate with myriad E3s, including BRCA1/BARD1 in DNA repair, CHIP in chaperone quality control, and MDM2 or PIRH2 in p53 turnover.