Active, Recombinant Human VHL / EloB / EloC Complex

Background: von Hippel-Lindau tumor suppressor (VHL) is a crucial gene involved in regulating cell growth and stability. It encodes the substrate recognition subunit of the cullin E3 ligase CRL-VHL. Under normal oxygen conditions, the VHL protein helps degrade hypoxia-inducible factors (HIFs), which are transcription factors that activate genes involved in angiogenesis, metabolism, and cell survival. When oxygen levels are sufficient, VHL binds to HIFs and tags them for destruction via the ubiquitin-proteasome pathway. VHL is widely used in targeted protein degradation strategies, particularly in the development of PROTACs™ (Proteolysis Targeting Chimeras). PROTACs™ are bifunctional molecules that simultaneously bind to a target protein and an E3 ubiquitin ligase, like VHL. When a PROTAC™, such as MZ1, recruits VHL to a specific target protein, VHL facilitates the ubiquitylation of that protein, marking it for destruction by the proteasome.