2 μg UBE2R1 run on 4-12% SDS-PAGE gel under reducing conditions, then visualized with Colloidal Coomassie Blue Stain.

Active, Recombinant Human UBE2R1

E2 Conjugating Enzymes

Product Code: TE2-012

Verified Applications: Recombinant Ubiquitin Conjugating Enzyme E2 R1 accepts activated ubiquitin from Ubiquitin Activating Enzyme 1 (an E1) in in vitro reactions. This charged E2 may subsequently transfer ubiquitin to a protein substrate in an E3 Ligase-catalyzed reaction. Appropriate enzyme concentrations are specific to the application.

Activity: Verified in Ubiquitin Charging Assay.

Purity: 95 %

Concentration: 50 μM

For Research Use Only (RUO)

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Note: To maintain product quality, larger quantities may be packaged as multiple smaller aliquots. The 1 mg size is generally supplied in a single vial.

UniProt #: P49427

Predicted Molecular Weight: 27 kDa

Tag: Untagged

Background and Additional Info

Background: UBE2R1 (also known as CDC34-like E2 enzyme, UBC3/UBE2R1) is an E2 ubiquitin-conjugating enzyme that mediates transfer of activated ubiquitin to substrate proteins in cooperation with E3 ligases. It plays a central role in the ubiquitin–proteasome system, regulating cell-cycle progression, signal transduction, and protein quality control by promoting polyubiquitylation and subsequent proteasomal degradation. UBE2R1 activity influences degradation of cyclins and CDK inhibitors, impacting G1/S transition and genomic stability. Dysregulation of UBE2R1 has been implicated in cancer and neurodegenerative disorders. Its enzymatic mechanism and interactions with specific E3s make it a candidate for targeted modulation in disease contexts.

Shipping: The product is shipped with dry ice. Upon receipt, store it immediately at the temperature recommended below.

Storage and Stability: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Aliquot and store ≤ -70°C (stable for 24 months from date of receipt).

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References: Sheng, Y., et al., (2012) Mol Cell Proteomics (8):329-41. PMID 22496338

Cen, B., et al., (2009) J Biol Chem 284(8):5265-5276. PMID 19112177

Semplici, F., et al., (2002) Oncogene 21(25):3978-398. PMID 12037680

Alternate Names: E2-32K, CDC34

Protein Sequence: GPGSMARPLVPSSQKALLLELKGLQEEPVEGFRVTLVDEGDLYNWEVAIFGPPNTYYEGGYFKARLKFPIDYPYSPPAFRFLTKMWHPNIYETGDVCISILHPPVDDPQSGELPSERWNPTQNVRTILLSVISLLNEPNTFSPANVDASVMYRKWKESKGKDREYTDIIRKQVLGTKVDAERDGVKVPTTLAEYCVKTKAPAPDEGSDLFYDDYYEDGEVEEEADSCFGDDEDDSGTEES