In Vitro Reconstituted Ubiquitylation Assays for Mechanistic Studies of E3 Ligases

In Vitro Reconstituted Ubiquitylation Assays for Mechanistic Studies of E3 Ligases Introduction Ubiquitylation is a widespread post-translational modification that regulates protein stability and cell signaling. Substrate specificity is conferred by more than 600 putative E3 ubiquitin ligases spanning several mechanistically distinct families. RING and U-box ligases position a ubiquitin-charged E2 relative to the substrate for […]