Recombinant K6-linked Tetra-Ubiquitin

Background: K6-linked polyubiquitin consists of multiple ubiquitin’s linked together between the C-terminal glycine of one ubiquitin and lysine 6 of the next. K6 linkages are less abundant than K48 or K63 chains and are formed by specific E2/E3 enzyme pairs. Functionally, K6 chains are implicated in mitochondrial quality control, mitophagy, DNA damage response, and regulation of protein-protein interactions rather than proteasomal degradation. They can recruit distinct ubiquitin-binding proteins and deubiquitylases (DUBs), producing signaling outcomes unique from other linkage types. complexity.