2 μg K6-linked Di-Ubiquitin run on 4-12% SDS-PAGE gel under reducing conditions
Human, Recombinant K6-linked Di-Ubiquitin
Ub/Ubl's
Product Code: TUB-046
Verified Applications: Ubiquitin chains exhibit diversity in length, linkage type, and associated cellular functions. K6-linked Di-Ubiquitin serves as a valuable reagent in assays involving ubiquitin-binding proteins and as a substrate for ubiquitin-specific deubiquitylating enzymes (DUBs).
Activity: Fully hydrolyzed by the K6-specific LotA-N deubiquitylase
Purity: 95%
Concentration: 58 μM
For Research Use Only (RUO)
Protein Name: K6-linked Di-Ubiquitin
UniProt #: Ub
Predicted Molecular Weight: 17 kDa
Background and Additional Info
Background: K6-linked polyubiquitin consists of multiple ubiquitin’s linked together between the C-terminal glycine of one ubiquitin and lysine 6 of the next. K6 linkages are less abundant than K48 or K63 chains and are formed by specific E2/E3 enzyme pairs. Functionally, K6 chains are implicated in mitochondrial quality control, mitophagy, DNA damage response, and regulation of protein-protein interactions rather than proteasomal degradation. They can recruit distinct ubiquitin-binding proteins and deubiquitylases (DUBs), producing signaling outcomes unique from other linkage types. complexity.
Shipping: The product is shipped with dry ice. Upon receipt, store it immediately at the temperature recommended below.
Storage and Stability: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Aliquot and store ≤ -20°C (stable for 24 months from date of receipt).
2 μg K6-linked Di-Ubiquitin run on 4-12% SDS-PAGE gel under reducing conditions