Active, Recombinant Human UBE2M

Background: UBE2M (also called UBC12) is a human E2 ubiquitin-conjugating enzyme specialized for the NEDD8 pathway. It catalyzes transfer of NEDD8, a ubiquitin-like modifier, from the E1 NEDD8-activating enzyme to substrate proteins, primarily cullin family scaffold proteins in Cullin-RING E3 ligases (CRLs). NEDD8 conjugation (neddylation) activates CRLs, promoting ubiquitin-dependent proteasomal degradation of many regulatory proteins involved in cell cycle, DNA repair, and signal transduction pathways. UBE2M works with E3 NEDD8 ligases (e.g., RBX1) and is regulated by expression levels, post-translational modifications, and interactions with deneddylases (e.g., CSN). Dysregulation of UBE2M/neddylation is implicated in cancer and other diseases.