Active, Recombinant Human UBE2D2

Background: Ubiquitin-Conjugating Enzyme E2 D2 (UBE2D2) is an E2 ubiquitin-conjugating enzyme that facilitates the transfer of ubiquitin from E1 activating enzymes to substrate proteins. This 152 amino acid protein is almost entirely UBC domain: an N-terminal α-helix for E3 docking, a four-stranded β-sheet, three α-helices and flexible catalytic loops. The NMR solution structure plus multiple RING-bound crystal complexes define an open ↔ closed continuum. UBE2D2 forms thioester-linked UBE2D2-ubiquitin conjugates that cooperate with myriad E3s, including TRAF6 in innate-immune signaling, CHIP in chaperone quality control and MDM2 or PIRH2 in p53 turnover.