2 μg AMSH* run on 4-12% SDS-PAGE gel under reducing conditions, then visualized with Colloidal Coomassie Blue Stain.
Active, Recombinant Human AMSH*
DUBs
Product Code: TDE-063
Verified Applications: Recombinant AMSH* is a ubiquitin-specific deconjugating fusion-enzyme that is highly specific for K63-linked polyubiquitin. Appropriate enzyme concentrations are specific to the application.
Activity: Verified in K63-linked Di-Ubiquitin Hydrolysis Assay.
Purity: 95 %
Concentration: 60-70 μM
For Research Use Only (RUO)
Protein Name: AMSH*
UniProt #: O95630
Predicted Molecular Weight: 41 kDa
Tag: Untagged
Background and Additional Info
Background: Human AMSH (also known as STAMBP) is a zinc-dependent JAMM/MPN⁺ deubiquitylase (DUB) whose ordered Ins-2 loop enforces K63-linked chain specificity. AMSH is only active when allosterically activated by its binding partners STAM1 or STAM2. Binding the SH3 domain of STAM within ESCRT-0 recruits AMSH to early endosomes. There it trims K63 ubiquitin signals just before multivesicular-body scission, thereby modulating ESCRT-regulated trafficking events. Loss-of-function mutations in AMSH cause autosomal-recessive microcephaly–capillary malformation syndrome, whereas pharmacologic or genetic inhibition of STAMBP dampens NLRP7/NALP7 inflammasome activation.
Formulation: 40 mM HEPES, 100 mM NaCl, 10% Glycerol, 1 mM EDTA, 1 mM TCEP, pH 7.6
Shipping: The product is shipped with dry ice. Upon receipt, store it immediately at the temperature recommended below.
Storage and Stability: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Aliquot and store ≤ -70°C (stable for 24 months from date of receipt).
Protein Sequence: GPGSTANPFEQDVEKATNEYNTTEDWSLIMDICDRVGSTPSGAKDCLKAIMKRVNHKVPHVALQALTLLGACVANCGKIFHLEVCSRDFATEVRSVIKNKAHPKVCEKLKSLMVEWSEEFQKDPQFSLISATIKSMKEEGVTFPSAGSQTVAAAAKNGTSLNKNKEDEDIAKAIELSLQEQKQQYTETGGSSGGSNSESIPTIDGLRHVVVPGRLCPQFLQLASANTARGVETCGILCGKLMRNEFTITHVLIPKQSAGSDYCNTENEEELFLIQDQQGLITLGWIHTHPTQTAFLSSVDLHTHCSYQMMLPESVAIVCSPKFQETGFFKLTDHGLEEISSCRQKGFHPHSKDPPLFCSCSHVTVVDRAVTITDLR
2 μg AMSH* run on 4-12% SDS-PAGE gel under reducing conditions, then visualized with Colloidal Coomassie Blue Stain.