Verified Applications: Recombinant Ubiquitin is activated for use in vitro by Recombinant Ubiquitin Activating Enzyme 1. This initial event in the ubiquitin-proteasome cascade requires Mg-ATP and ubiquitin. Appropriate protein concentrations are specific to the application.
2 ug UBA1 run on 4-12% SDS-PAGE gel under reducing conditions, then stained with colloidal Coomassie Blue
Formulation: 10 mM HEPES, pH 7.6
Shipping: The product is shipped with cold packs or dry ice. Upon receipt, store it immediately at the temperature recommended below.
Storage and Stability: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Aliquot and store ≤ -20°C (stable for 48 months from date of receipt).
Protein Sequence: MQIFVKTLTGKTITLEVEPSDTIENVKAKIQDKEGIPPDQQRLIFAGKQLEDGRTLSDYNIQKESTLHLVLRLRGG
Background Information and Alternate Names
Ubiquitin is a small regulatory protein that plays critical roles in various cellular processes in eukaryotic organisms. Its primary function is to tag proteins for degradation. This process, known as ubiquitylation, involves the attachment of ubiquitin molecules to substrate proteins, signaling them for destruction by the proteasome. Additionally, ubiquitylation also regulates many other cellular functions, including DNA repair, cell cycle regulation, and response to stress.
