2 μg OTUB1* run on 4-12% SDS-PAGE gel under reducing conditions, then visualized with Colloidal Coomassie Blue Stain.

Active, Human, Recombinant OTUB1*

DUBs

Product Code: TDE-062

Verified Applications: Recombinant human OTUB1* is a Ubiquitin-specific deconjugating fusion-enzyme that is highly specific for K48-linked poly-ubiquitin. Appropriate enzyme concentrations are specific to the application.

Activity: Verified in K48-linked Di-ubiquitin Hydrolysis Assay.

Purity: 95%

Concentration: 25 – 35 μM

For Research Use Only (RUO)

Protein Name: OTUB1*

UniProt #: Q96FW1

Predicted Molecular Weight: 47

Background and Additional Info

Background: Human ubiquitin thioesterase OTUB1 (Otubain 1, OTUB1) belongs to the ovarian tumor (OTU) family of deubiquitylases (DUBs). Human OTUB1 (271 amino acids) comprises an N-terminal helical extension that binds ubiquitin-charged E2 complexes, followed by the catalytic OTU domain housing the Cys91–His265–Asp268 triad. Crystal structures of OTUB1 in complex with UBE2D-ubiquitin (PDB 4DDI) reveal how the N-terminal helix and distal S1/S1′ pockets cooperate with the E2 backside to enforce OTUB1’s K48-linked poly-ubiquitin specificity.

Formulation: 40 mM HEPES, 100 mM NaCl, 10% Glycerol, 1 mM EDTA, 1 mM TCEP, pH 7.6

Shipping: The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.

Storage and Stability: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Aliquot and store ≤ -70°C (stable for 24 months from date of receipt).

Download the Datasheet

Additional Product Information

Alternate Names: OTB1, OTU1); The “star” suffix denotes fusion to UBE2D1(C85A), an inert isoform of the ubiquitin-conjugating enzyme UBC H5a.

Protein Sequence: GPGSMALKRIHKELNDLARDPPAQCSAGPVGDDMFHWQATIMGPNDSPYQGGVFFLTIHFPTDYPFKPPKVAFTTRIYHPNINSNGSIALDILRSQWSPALTISKVLLSICSLLCDPNPDDPLVPEIARIYKTDREKYNRIAREWTQKYAMGGSSGGSSGGSDSEGVNCLAYDEAIMAQQDRIQQEIAVQNPLVSERLELSVLYKEYAEDDNIYQQKIKDLHKKYSYIRKTRPDGNCFYRAFGFSHLEALLDDSKELQRFKAVSAKSKEDLVSQGFTEFTIEDFHNTFMDLIEQVEKQTSVADLLASFNDQSTSDYLVVYLRLLTSGYLQRESKFFEHFIEGGRTVKEFCQQEVEPMCKESDHIHIIALAQALSVSIQVEYMDRGEGGTTNPHIFPEGSEPKVYLLYRPGHYDILYK