Activity: Polyubiquitin chains can serve as substrates in in vitro reactions with deubiquitylating enzymes (DUBs) that hydrolyze bonds between adjacent ubiquitin molecules. Furthermore, polyubiquitin chains can be utilized to explore the mechanisms of binding and recognition between these chains and other proteins with UIMs, UBAs, and or other ubiquitin-binding motifs.
Purity: 95%
Concentration: 58 uM
For Research Use Only (RUO)
Protein Name: K11-linked Di-Ubiquitin
UniProt #: Ub
Predicted Molecular Weight: 17 kDa
Background and Additional Info
Background: K11-linked ubiquitin chains are formed when the C-terminal glycine of one ubiquitin is conjugated to lysine 11 of another, producing homotypic or mixed polyubiquitin chains. They are assembled primarily by E2 enzyme UBE2S in conjunction with APC/C. K11 linkages target substrates for proteasomal degradation, especially during cell cycle progression, controlling levels of cyclins and mitotic regulators. They also regulate endoplasmic reticulum-associated degradation (ERAD) and signaling pathways by modulating substrate fate and interactions with ubiquitin receptors that distinguish linkage types. Deubiquitylases such as Cezanne (OTUD7B) disassemble K11 chains, fine-tuning cellular proteostasis.
Shipping: The product is shipped with dry ice. Upon receipt, store it immediately at the temperature recommended below.
Storage and Stability: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. Aliquot and store ≤ -20°C (stable for 24 months from date of receipt).
