Active, Recombinant Human SENP1 (411-644)

Background: SENP1 (sentrin/SUMO-specific protease 1) is a cysteine protease that reverses SUMOylation by processing SUMO precursors and deconjugating SUMO from target proteins. Structurally, it has an N-terminal regulatory region and a conserved C-terminal catalytic domain. By removing SUMO modifications, SENP1 modulates the activity, stability, and localization of transcription factors, signaling proteins, and DNA-repair factors, thereby influencing cell-cycle progression, apoptosis, and stress responses. SENP1 is upregulated by hypoxia and can enhance hypoxic signaling (for example, via HIF‑1α), and its dysregulation is linked to tumorigenesis and metastasis. In vitro, SENP1 is useful in removing SUMO fusion tags from recombinant proteins, or for de-SUMOylating proteins in lysates.